Sensibilität von Seefischen an Bord. Teil 3: Untersuchungen an demersalen Fischarten der Barentssee

In previous papers the sensibility of pelagic and demersal fishes caught at depth of up to 80 m was reported. This paper deals with the sensitiveness of flatfishes, gadids, and redfish caught at depth between 260 and 450 m and with trawling times between 1 and 6 h. The sensitiveness of the fishes was tested according to the method described in previous publications (Münkner et. al. 1998) after 10 min keeping in running sea water and after 1h bulk storage respectively. The sensitiveness of the fishes increased from cod to saithe to haddock. Surprisingly American plaice and Greenland halibut turned out to be very sensitive, far more sensitive than plaice and dab caught at lower depths in the North Sea. This was indicated by the high amount of animals showing rigor already after a trawling time of 2 hand 10 min of keeping in seawater. After 1 h of bulk storage and increasing trawling time sensitiveness of all fishes decreased, as expected, significantly. Besides mechanical encroachments the main problem for the fishes caught at greater depths was the gas supersaturation in the blood and tissue causing blockage of the gill capillary vessels, exophthalmus, visible gas bubbles in the skin and eyes, and in some cases protusion of the intestines through the snout due to rapid dilatation of the swimbladder.

Analysis of soils and sediments for organic pollutants

20 samples of soil or sediment (7 of which were predominantly sand) from various locations were received for analysis of their content of organic pollutants. These analyses were performed using a capillary column gas chromatograph equipped with an electron impact (E.I.) mass spectrometer as detector and using computerised data storage. In addition to the target compounds, the full scan data were examined to determine the composition of natural organic products and a series of diagnostic fragment ions was used to search for additional anthropogenic products. Organic-rich environmental samples are notoriously difficult to analyse for pollutant organics owing to the presence of high concentrations of many natural organic compounds. A single procedure for extraction and clean-up was adopted. It was designed for chlorinated aliphatic and aromatic hydrocarbons and other pesticides containing acidic functional groups and was based on published methods for the determination of organic pollutants in soils and sediments. 4 soils and 2 sands showed levels of one or two groups of PCBs slightly in excess of the detection limit, one sample showed a similar level of 2,4-D and 3 samples contained dieldrin at or just above the detection limit.

ReefBase - a global database of coral reef systems and their resources

ReefBase a global database of coral reefs systems and their resources was initiated at International Center for Living Aquatic Resources Management (ICLARM), Philippines in November 1993. The CEC has provided funding for the first two years and the database was developed in collaboration with the World Conservation Monitoring Centre in Cambridge, UK, as well as other national, regional, and international institutions. The ReefBase project activities and what ICLARM will do to accomplish the project objectives are briefly discussed.

Carbonyls from some commercially important fishes and shell fishes of tropical waters

Results of a preliminary study of the hexane extractable carbonyls from three fish and one shell fish are reported. Volatile carbonyls that can be isolated from oil sardine by distillation at different temperatures in air or nitrogen atmosphere were also studied. Carbonyls were converted to their 2:4 dinitro phenyl hydrozones. These were fractionated by column chromatography and the different fractions were analysed by capillary gas chromatography. The data show wide variations in the content of different carbonyls in different fishes. Carbonyls in distillates from muscle water homogenates of oil sardine showed some differences from the carbonyls extracted with hexane from the same fish. More data is necessary to correlate the carbonyls with the characteristic flavour of each fish.

Effect of preprocessing storage conditions on the carbonyls of oil sardine

Changes in the total as well as major individual carbonyls of oil sardine muscle during storage at room temperature for 24 h and in crushed ice up to 6 days are reported. Carbonyls extracted with hexane were converted to their 2:4 dinitrophenyl hydrazone (DNPH) derivatives and were separated into major classes by column chromatography on celite/magnesia. Individual carbonyls were then identified by capillary gas chromatography of these derivatives. Though absolute values for carbonyls exhibited wide variations depending upon the degree of freshness, the pattern of changes in the carbonyls during storage of fish under different conditions gave an insight into the influence of carbonyls on flavour. The significance of the findings is discussed.

Effect of cooking and drying on carbonyls of oil sardine

Changes in the total as well as major individual carbonyls of oil sardine during steam cooking, oven drying, sun drying and freeze drying are presented. Carbonyls extracted with hexane were converted to their 2:4 dinitro phenyl hydrazone (DNPH) derivatives and were separated into major classes by column chromatography on celite/magnesia. Individual carbonyls were then identified by capillary gas chromatography of the DNPH derivatives. Dehydration and heating increase the carbonyl production from highly unsaturated fish lipids. The carbonyls produced react with other muscle constituents leading to complex changes. The influence of the mode of dehydration on these different aspects and their net effect on flavour are discussed.